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Comparative Study
. 2010;54(4):e32.
doi: 10.4081/ejh.2010.e32.

Koilocytes are enriched for alkaline-labile sites

E I Cortés-Gutiérrez  1 , M I Dávila-Rodríguez, J L Fernández, C López-Fernández, J Gosálvez
Affiliations
Comparative Study

Koilocytes are enriched for alkaline-labile sites

E I Cortés-Gutiérrez et al. Eur J Histochem. 2010.

Abstract

This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection.

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Figures

Figure 1
Figure 1
Papanicolaou-stained cervix epithelial cells showing propidium iodide (PI), DBD-FISH, and comet assay of normal cells, binucleate/multinucleate koilocyes, demonstrating the percentage of koilocytes (binucleate and multinucleate) in the Papanicolaou stain, the halo of hybridization in DBD-FISH, and a comet assay on one specimen with CIN 1.
See this image and copyright information in PMC

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