Phylogeny and morphology of four new species of Lasiodiplodia from Iran

Abstract

Four new species of Lasiodiplodia; L. citricola, L. gilanensis, L. hormozganensis and L. iraniensis from various tree species in Iran are described and illustrated. The ITS and partial translation elongation factor-1α sequence data were analysed to investigate their phylogenetic relationships with other closely related species and genera. The four new species formed well-supported clades within Lasiodiplodia and were morphologically distinct from all other known species.

Keywords: Botryosphaeriaceae; EF-1α; ITS; Lasiodiplodia; phylogeny; taxonomy.

Fig. 1

One of six most parsimonious trees obtained from combined ITS and EF-1α sequence data. MP and NJ bootstrap values are given based on 1 000 pseudoreplicates above and below the branches respectively. The tree is rooted to Dothiorella sarmentorum (CBS115038) and Spencermartinsia sp. (CBS117006).

Fig. 2

Lasiodiplodia citricola holotype. a. Conidiomata on pine needles in culture; b. conidia developing on conidiogenous cells between paraphyses; c. annellations; d, e. paraphyses; f. hyaline, immature conidia; g, h. mature conidia in two different focal planes to show the longitudinal striations. — Scale bars: a = 1 000 μm; b, c = 5 μm; d–h = 10 μm.

Fig. 3

Lasiodiplodia gilanensis holotype. a. Conidiomata on pine needles in culture; b–d. conidia developing on conidiogenous cells between paraphyses; e. paraphyses; f, g. hyaline, immature conidia; h, i. mature conidia in two different focal planes to show the longitudinal striations. — Scale bars: a = 1 000 μm; b, c = 5 μm; d–i = 10 μm.

Fig. 4

Lasiodiplodia hormozganensis holotype. a. Conidiomata on pine needles in culture; b, c. conidia developing on conidiogenous cells between paraphyses; d, e. paraphyses; f. hyaline immature conidia; g, h. mature conidia in two different focal planes to show the longitudinal striations. — Scale bars: a = 1 000 μm; b, c = 5 μm; d–h = 10 μm.

Fig. 5

Lasiodiplodia iraniensis holotype. a. Conidiomata on pine needles in culture; b, c. conidia developing on conidiogenous cells between paraphyses; d, e. paraphyses; f. hyaline, immature conidia; g, h. mature conidia in two different focal planes to show the longitudinal striations. — Scale bars: a = 500 μm; b, c = 5 μm; d–h = 10 μm.