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. 2011 Feb;14(2):141-5.
doi: 10.3779/j.issn.1009-3419.2011.02.07.

[Changes on mitochondrial DNA content in non-small cell lung cancer]

[Article in Chinese]
Hongmei Wang  1 , Jigang Dai
Affiliations

[Changes on mitochondrial DNA content in non-small cell lung cancer]

[Article in Chinese]
Hongmei Wang et al. Zhongguo Fei Ai Za Zhi. 2011 Feb.

Abstract
in English, Chinese

Background and objective: It has been proven that the mitochondrial DNA (mtDNA) mutations and content change were associated with increasing risk of tumorigenesis. MtDNA content is significantly reduced in most substantive tumors. The aim of this study is to demonstrate whether mtDNA content is positively associated with non-small cell lung cancer (NSCLC) risk.

Methods: MtDNA content in 37 matched lung carcinoma and histologically adjacent normal lung tissue samples from patients were analyzed by fluorogenic 5-nuclease real-time PCR techniques.

Results: The mean copy number of mtDNA in lung carcinoma tissue samples was statistically lower than that in adjacent histologically normal lung tissue samples (P < 0.001).

Conclusions: The change of mtDNA content may play an important role in NSCLC.

背景与目的: 已有的研究表明：线粒体DNA（mitochondrial DNA, mtDNA）突变和拷贝数的改变和肿瘤有着密切联系；大多数实体性肿瘤中mtDNA拷贝数有明显降低。本研究旨在探讨线粒体基因组含量的改变和原发性非小细胞肺癌（non-small cell lung cancer, NSCLC）的关系。

方法: 通过实时荧光定量PCR，对肺癌及相对应的癌旁肺组织mtDNA的含量进行精确定量（拷贝数/细胞）。

结果: 肺癌组织mtDNA的平均拷贝数/细胞为395±125，而相对应的正常肺组织为733±196，前者明显低于后者（P < 0.001）。肺癌mtDNA含量的改变与患者性别、年龄、是否吸烟及肿瘤的病理类型无关（P > 0.05）。

结论: mtDNA含量的改变与NSCLC的发生发展密切相关，同时也可能影响其治疗和预后。

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Figures

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mtDNA HV1（图 1A，图 1B）和核基因（图 1C，图 1D）实时荧光定量PCR动力曲线图和标准曲线 Amplification plots and standard curves of real-time PCR for Mitochondrial DNA HV1 (Fig 1A, Fig 1B) and nuclear β-globin gene (Fig 1C, Fig 1D)
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肺癌及相对应的癌旁肺组织mtDNA拷贝数散点图及线性相关分析 MtDNA copy number in lung cancinoma and adjacent nomal samples
See this image and copyright information in PMC

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