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. 2011 Feb;14(2):146-50.
doi: 10.3779/j.issn.1009-3419.2011.02.08.

[Expression and clinical significance of ZO-1 in patients with non-small cell lung cancer]

[Article in Chinese]
Yekai Wang  1 , Jihang Zhou, Fang Zeng, Yanyan Huang, Shiquan Zhou, Xiaoguang Liu
Affiliations

[Expression and clinical significance of ZO-1 in patients with non-small cell lung cancer]

[Article in Chinese]
Yekai Wang et al. Zhongguo Fei Ai Za Zhi. 2011 Feb.

Abstract
in English, Chinese

Background and objective: It has been proven that the expression of ZO-1 (zonula occluden-1) was correlated with the growth and metastasis of cancer. The aim of this study is to investigate the relationship between ZO-1 gene expression and clinicopathologic parameters and the survival rates in the patients with non-small cell lung cancer (NSCLC).

Methods: The expression levels of ZO-1 gene in 101 patients with NSCLC and lung tissues of 61 patients with benign lung disease were detected by real-time PCR, Western blot and immunohistochemical techniques.

Results: There was a significant statistic difference of ZO-1 mRNA expression levels between carcinoma group and control group (P < 0.01). In carcinoma group, adjacent group and control group, ZO-1 protein expression levels were with significantly statistic difference between each other (P < 0.01 or P < 0.05). There was a significantly statistic difference between ZO-1 mRNA expression levels in survival during 2 years follow-up group and death during 2 years follow-up group (t=-5.61, P < 0.01).

Conclusions: ZO-1 is a valuable predictive marker for NSCLC patients.

背景与目的: 已有的研究表明紧密连接蛋白-1（zonula occluden-1, ZO-1）表达和肿瘤细胞的生长和转移存在密切联系。本研究旨在探讨非小细胞肺癌（non-small cell lung cancer, NSCLC）组织中ZO-1表达的临床意义。

方法: 应用实时荧光定量PCR、Western blot和免疫组化检测101例NSCLC癌组织及癌旁组织中ZO-1 mRNA和蛋白表达，以61例肺部良性病变患者作为对照。

结果: ZO-1 mRNA在癌组织和肺良性病变组织间差异具有统计学意义（P＜0.01）。Western blot显示：ZO-1蛋白在癌组织、癌旁组织、肺良性病变组织的相对表达量之间均存在明显差异（P＜0.01或P＜0.05）。免疫组化显示：ZO-1平均光密度在癌组织、癌旁组织和肺良性病变组织中分别为69.55± 17.13、246.39±83.15、330.93±72.44，组间差异均具有统计学意义（P＜0.01）。伴有淋巴结转移与不伴淋巴结转移肺癌组织ZO-1 mRNA表达水平比较有显著性差异（t=-5.07, P＜0.01），肺癌患者术后2年内生存组和2年内死亡组间ZO-1 mRNA表达水平比较差异具有统计学意义（t=5.61, P＜0.01）。

结论: ZO-1 mRNA和蛋白在NSCLC癌组织中表达降低，并与淋巴结转移的术后生存有密切关系。

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Figures

1
1
肺癌患者癌组织、癌旁组织和对照组中ZO-1蛋白表达。A：Wester blot检测ZO-1表达；B：柱状分析图表明ZO-1表达在癌组织、癌旁组织和对照组中的差异存在明显差异；泳道1-2：癌旁组织；泳道3-4：对照组；泳道5-6：癌组织；*：与对照组相比，P＜0.05；**：与对照组相比，P＜0.01；##：与癌旁组相比，P＜0.01。 Expression of ZO-1 protein in carcinoma tissue group, adjacent tissue group and control group. A: Expression of ZO-1 protein determined by Western blot; B: The densitometric analysis reveal that the levels of ZO-1 protein among the carcinoma tissue group, the adjacent tissue group and the control group were with significantly statistic difference; Lane 1-2: adjacent tissue group; Lane 3-4: control group; Lane 5-6: carcinoma tissue group; *: compared with the control group, P < 0.05; **: compared with the control group, P < 0.01; ##: compared with the adjacent tissue group, P < 0.01.
2
2
NSCLC患者肺组织中ZO-1免疫组化。A：ZO-1在肺组织中的免疫组化（SP，×20）；B：柱状分析图表明ZO-1表达在癌组织、癌旁组织和对照组中的差异存在显著统计学意义；**：与对照组相比，P＜0.01；##：与癌旁组相比，P＜0.01。 Immunohistochemical assessmen of ZO-1 in lung tissue of patients with NSCLC. A: Immunohistochemical assessmen of ZO-1 in lung tissue (SP, ×20). In adjacent tissue of gland cancer and control group, ZO-1 distributed through the alveolar walls completely. But in carcinoma tissue of gland cancer, ZO-1 distributed through the alveolar walls incompletely; there is a low expression of ZO-1 between the gland cancer cells; B: The densitometric analysis reveal that the averages of the optical densities of ZO-1 protein among the carcinoma tissue group, the adjacent tissue group and the control group were significant statistic different; **: compared with the control group, P < 0.01; ##: compared with the adjacent tissue group, P < 0.01.
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