Liolope copulans (Trematoda: Digenea: Liolopidae) parasitic in Andrias japonicus (Amphibia: Caudata: Cryptobranchidae) in Japan: Life cycle and systematic position inferred from morphological and molecular evidence

Abstract

The life cycle of Liolope copulans Cohn, 1902 (Trematoda: Digenea: Liolopidae), an intestinal parasite of the Japanese giant salamander Andrias japonicus (Temminck) (Amphibia: Caudata: Cryptobranchidae), was studied in the field and laboratory in Japan. This is the first description of mother sporocyst, daughter sporocyst and cercariae of a liolopid species. Non-oculate longifurcate pharyngeate cercariae were formed in lanceolate-cylindrical daughter sporocysts in Semisulcospira libertina (Gould) (Gastropoda: Sorbeoconcha: Pleuroceridae). They successfully developed to encapsulated metacercariae in cyprinid fishes, Nipponocypris sieboldii (Temminck and Schlegel) and Rhynchocypris lagowskii (Dybowski), by experimental infection. Cercariae had a V-shaped excretory vesicle with two looped arms, as in metacercariae and adults. Developmental stages from mother sporocyst to adult are described and illustrated. DNA sequencing was conducted for 28S and 18S rDNA of mother and daughter sporocysts, cercariae, and an adult. The result of molecular phylogenetic analysis suggests that L. copulans may be one of the basal taxa of the order Diplostomida Olson, Cribb, Tkach, Bray, and Littlewood, 2003, but its systematic position is still unclear because of the topological inconsistence between the 28S and 18S trees. Therefore, we tentatively place the family Liolopidae in the superfamily Diplostomoidea, mainly based on the morphology of sporocysts and cercariae.