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. 2011 May;49(5):1961-4.
doi: 10.1128/JCM.02321-10. Epub 2011 Feb 23.

Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ingrid Wybo  1 , Annelies De Bel, Oriane Soetens, Fedoua Echahidi, Kristof Vandoorslaer, Marina Van Cauwenbergh, Denis Piérard
Affiliations

Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ingrid Wybo et al. J Clin Microbiol. 2011 May.

Abstract

Carbapenem resistance in Bacteroides fragilis is associated with cfiA-encoded class B metallo-beta-lactamase. cfiA-negative and cfiA-positive isolates belong to genotypically distinct groups. Of a total of 248 B. fragilis isolates included in this study, 214 were susceptible, 10 were intermediate, and 24 were resistant to meropenem. We show that matrix-assisted laser desorption ionization-time of flight mass spectrometry is able to differentiate between cfiA-negative and cfiA-positive isolates and predict carbapenem resistance in a routine laboratory setting.

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Figures

Fig. 1.
Fig. 1.
The composite correlation index (CCI) values between cfiA-positive (1 to 4) and cfiA-negative (5 to 8) isolates were higher than CCI values if cfiA-positive isolates were compared with cfiA-negative isolates.
Fig. 2.
Fig. 2.
Dendrogram of all tested isolates. cfiA-positive B. fragilis isolates are represented in red, and cfiA-negative isolates are in blue.
See this image and copyright information in PMC

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