Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis
- PMID: 21353953
- DOI: 10.1016/j.diagmicrobio.2010.10.003
Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis
Abstract
The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species (C. albicans, C. glabrata, C. kefyr, C. parapsilosis, and C. guilliermondii). HRMA was able to differentiate clinical Candida species and resulted in being sensitive, reproducible, and inexpensive.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
-
The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis.Mol Oral Microbiol. 2014 Jun;29(3):117-30. doi: 10.1111/omi.12050. Epub 2014 Apr 12. Mol Oral Microbiol. 2014. PMID: 24628973
-
Real-time LightCycler polymerase chain reaction and melting temperature analysis for identification of clinically important Candida spp.J Microbiol Immunol Infect. 2009 Aug;42(4):290-5. J Microbiol Immunol Infect. 2009. PMID: 19949751
-
PCR-based identification of pathogenic Candida species using primer mixes specific to Candida DNA topoisomerase II genes.Yeast. 2002 Aug;19(11):973-89. doi: 10.1002/yea.892. Yeast. 2002. PMID: 12125054
-
Candida albicans or Candida dubliniensis?Mycoses. 2011 Jan;54(1):1-16. doi: 10.1111/j.1439-0507.2009.01759.x. Mycoses. 2011. PMID: 19682314 Review.
-
Identification of Candida species in the clinical laboratory: a review of conventional, commercial, and molecular techniques.Oral Dis. 2014 May;20(4):329-44. doi: 10.1111/odi.12123. Epub 2013 May 16. Oral Dis. 2014. PMID: 23679136 Review.
Cited by
-
Molecular identification of Candida isolates by Real-time PCR-high-resolution melting analysis and investigation of the genetic diversity of Candida species.J Clin Lab Anal. 2020 Oct;34(10):e23444. doi: 10.1002/jcla.23444. Epub 2020 Jul 12. J Clin Lab Anal. 2020. PMID: 32656934 Free PMC article.
-
Evaluation of a Novel Mitochondrial Pan-Mucorales Marker for the Detection, Identification, Quantification, and Growth Stage Determination of Mucormycetes.J Fungi (Basel). 2019 Oct 11;5(4):98. doi: 10.3390/jof5040098. J Fungi (Basel). 2019. PMID: 31614610 Free PMC article.
-
High-resolution melting analysis assay for identification of Fonsecaea species.J Clin Lab Anal. 2018 Feb;32(2):e22257. doi: 10.1002/jcla.22257. Epub 2017 May 22. J Clin Lab Anal. 2018. PMID: 28543666 Free PMC article.
-
Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.J Clin Microbiol. 2016 May;54(5):1295-303. doi: 10.1128/JCM.00118-16. Epub 2016 Mar 2. J Clin Microbiol. 2016. PMID: 26935732 Free PMC article.
-
Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice.Jundishapur J Microbiol. 2015 May 31;8(5):e18984. doi: 10.5812/jjm.8(5)2015.18984. eCollection 2015 May. Jundishapur J Microbiol. 2015. PMID: 26060567 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources