[Effect of extracellular matrix and serum components on cellular adhesion and growth in vitro and in vivo]

Abstract

The recent cell-biological research have been reported the usefulness of extracellular matrix, such as collagen and fibronectin, in cell culture techniques and in vitro experiments. These matrix began to applied in medical treatments and brought many success. Fibroblast and osteoblast have characteristics to promote stabilization of implant materials in vivo. These cells revealed the additional effects to control mending and healing of surgical invasion. The role of extracellular matrix and serum components for cellular adhesion and growth have been discussed extensively. The recent studies reported that collagen and other materials promoted the cellular behavior. The author discussed the effect of extracellular matrix and serum components or the established cell lines on cellular adhesion and growth, which were performed in vitro and in vivo. The following cell lines were employed in this study, fibroblastic cell lines from mouse (3T12-3) and human (SF-TY), osteogenic cell line from mouse (MC3T3-E1) and osteogenic cell lines from human (SAOS-2, NY), osteoblastic cell line (HuO-3N1) and rabbit cell line. Different types of collagen were used as the coating material on glass surface by the method of collagen-gel culture method. Fetal calf serum, bovine serum albumin, fibronectin and proteoglycan were also employed in this study. After the cultivation, adherent cells were stained by Gimsa's method and counted microscopically. In cellular-adhesion test, the most cell number was found on the collagen type I coated glass surface to compare with the other materials, such as fetal calf serum, bovine serum albumin, proteoglycan, employed in all the tested cell lines. Growth of the defined cell-number on glass surface was compared among used materials, and all the osteogenic cell lines and fibroblastic cell lines were found to grow well on collagen type I-fibronectin, collagen type I, and fibronectin coated glass surface to compare with other materials employed in this study. Collagen-fibronectin and collagen-coated alumina ceramic tubes were embedded in the mandibular bones of rabbits, and the aspect was observed with the passage of time. These treatments promoted the osseous area in the tubes. These findings strongly indicated that surface-coated biomaterial by collagen and collagen-fibronectin enhanced cellular adhesion, and promoted the growth of fibroblast and osteoblast in vitro and in vivo.