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. 2007 Jul 1:2007:pdb.prot4786.
doi: 10.1101/pdb.prot4786.

SNP Mining from Maize 454 EST Sequences

W Brad Barbazuk  1 , Scott Emrich, Patrick S Schnable
Affiliations

SNP Mining from Maize 454 EST Sequences

W Brad Barbazuk et al. CSH Protoc. .

Abstract

INTRODUCTIONIn this protocol, 454 expressed sequence tags (ESTs) are generated by sequencing shoot apical meristem (SAM) cDNA from maize inbred lines on the 454 Life Sciences GS-20 sequencing system. The computational tool PolyBayes (Marth et al. 1999) is then used to identify single-nucleotide polymorphisms (SNPs). PolyBayes has been used successfully to identify SNPs in many different systems, including maize, and is particularly recommended for identifying SNPs in 454 sequences.

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