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. 2007 Sep 1:2007:pdb.prot4826.
doi: 10.1101/pdb.prot4826.

Drosophila embryo dechorionation

Wendy F Rothwell, William Sullivan

Drosophila embryo dechorionation

Wendy F Rothwell et al. CSH Protoc. .

Abstract

INTRODUCTIONEarly Drosophila embryos are particularly amenable to cellular analysis. However, they are protected by an outer chorion layer, as well as an impermeable and opaque vitelline membrane. Consequently, preparation of Drosophila embryos for fixed-sample analysis must include procedures for chorion removal, vitelline membrane permeabilization, fixation, and finally, vitelline membrane removal. This protocol describes a method for removing the chorion layer.

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