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. 2011 Mar;8(3):192-4.
doi: 10.1038/nmeth0311-192.

Recovery of intact DNA nanostructures after agarose gel-based separation

Gaëtan Bellot, Mark A McClintock, Chenxiang Lin, William M Shih

Recovery of intact DNA nanostructures after agarose gel-based separation

Gaëtan Bellot et al. Nat Methods. 2011 Mar.
No abstract available

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Figures

Figure 1
Figure 1. Agarose-gel analysis and transmission electron microscopy (TEM) of various DNA origami after gel purification
i. Cylinder model of the DNA nanostructures, with each cylinder representing a DNA double helix. (a), Six-helix bundle, (b), Twelve-helix bundle. (c), Six-helix bundle ring. (d), Prestressed tensegrity-structure kite. ii, kb ladder. iii, Unpurified DNA nanostructures, arrows indicate the region of each lane that was extracted from the gel during purification before TEM imaging. iv, 30% sucrose-gel-purified nanostructures. v, Pellet-pestle homogenization recovered gel-purified nanostructures. ImageJ was used for gel-image analysis to estimate the yield of purification (bottom of each lane). vi, TEM micrographs of the nanostructures after 30% sucrose gel purification. Lane images for each object cropped from a single gel.
See this image and copyright information in PMC

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