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. 1990 Jan;9(1):251-5.
doi: 10.1002/j.1460-2075.1990.tb08102.x.

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes

A Bindereif  1 , T Wolff, M R Green
Affiliations

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes

A Bindereif et al. EMBO J. 1990 Jan.

Abstract

U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

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References

    1. Nature. 1986 Nov 27-Dec 3;324(6095):341-5 - PubMed
    1. Cell. 1987 Jun 19;49(6):763-74 - PubMed
    1. EMBO J. 1987 Aug;6(8):2415-24 - PubMed
    1. Genes Dev. 1987 Nov;1(9):1014-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Jan;85(2):411-5 - PubMed

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