Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar 2;16(3):2075-83.
doi: 10.3390/molecules16032075.

In vitro inhibitory effects of cyandin-3-rutinoside on pancreatic α-amylase and its combined effect with acarbose

Sarinya Akkarachiyasit  1 , Sirintorn Yibchok-Anun, Sumrit Wacharasindhu, Sirichai Adisakwattana
Affiliations

In vitro inhibitory effects of cyandin-3-rutinoside on pancreatic α-amylase and its combined effect with acarbose

Sarinya Akkarachiyasit et al. Molecules. .

Abstract

The inhibitory activity on pancreatic α-amylase by cyanidin-3-rutinoside was examined in vitro. The IC₅₀ value of cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The structure of cyanidin-3-rutinoside.
Figure 2
Figure 2
Lineweaver-Burk plots for inhibitory activity of C3R on pancreatic α-amylase.
Figure 3
Figure 3
The combined effect of acabose and C3R (1 μM) on inhibition of pancreatic α-amylase. Result are expressed as means ± S.E.M; n = 3. *P < 0.01 compared with acarbose (3.12 μM) and #P < 0.01 compared with acarbose (6.25 μM).
See this image and copyright information in PMC

References

    1. Tundis R., Loizzo M.R., Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: An update. Mini Rev. Med. Chem. 2010;10:315–331. doi: 10.2174/138955710791331007. - DOI - PubMed
    1. Chiasson J.L., Josse R.G., Gomis R., Hanefeld M., Karasik A., Laakso M. STOP-NIDDM trial research group. Acarbose for the prevention of Type 2 diabetes, hypertension and cardiovascular disease in subjects with impaired glucose tolerance: Facts and interpretations concerning the critical analysis of the STOP-NIDDM Trial data. Lancet. 2002;359:2072–2077. doi: 10.1016/S0140-6736(02)08905-5. - DOI - PubMed
    1. Kalt W., Hanneken A., Milbury P., Tremblay F. Recent research on polyphenolics in vision and eye health. J. Agric. Food Chem. 2010;58:4001–4007. doi: 10.1021/jf903038r. - DOI - PubMed
    1. Laokuldilok T., Shoemaker C.F., Jongkaewwattana S., Tulyathan V. Antioxidants and antioxidant activity of several pigmented rice brans. J. Agric. Food Chem. 2011;59:193–199. doi: 10.1021/jf103649q. - DOI - PubMed
    1. Wang L.S., Stoner G.D. Anthocyanins and their role in cancer prevention. Cancer Lett. 2008;269:281–290. doi: 10.1016/j.canlet.2008.05.020. - DOI - PMC - PubMed

Publication types

LinkOut - more resources