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. 2011 Jun;50(6):1056-8.
doi: 10.1016/j.yjmcc.2011.02.015. Epub 2011 Mar 5.

Homeodomain only protein x is down-regulated in human heart failure

Chinmay M Trivedi  1 , Thomas P Cappola, Kenneth B Margulies, Jonathan A Epstein
Affiliations

Homeodomain only protein x is down-regulated in human heart failure

Chinmay M Trivedi et al. J Mol Cell Cardiol. 2011 Jun.

Abstract

Homeodomain only protein x (Hopx) is an unusual homeodomain protein that has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. In the present study, we examined the expression profile of Hopx in various models of pathologic cardiac hypertrophy and failure. Hopx expression is significantly reduced in neonatal rat cardiac myocytes after α/β adrenergic receptor agonist treatment. Cardiac hypertrophy and failure induced by transaortic constriction in mice causes marked down-regulation of Hopx expression. Interestingly, HOPX expression was significantly reduced in hearts of humans with end-stage heart failure when compared to non-failing control hearts, and HOPX levels remain low after LVAD support. Our findings suggest that HOPX/Hopx expression is reduced in multiple examples of human and murine cardiac hypertrophy and failure.

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Figures

Figure 1
Figure 1
Downregulation of Hopx expression in response to hypertrophic stimuli in vitro and in vivo: (A-C) Hopx or Nppa transcripts were detected by qRT-PCR from cultured neonatal rat cardiomyocytes treated with or without isoproterenol or angiotensin II for 48 hours (mean ± SEM, n=3). Gapdh serves as control. (D-E) Transcripts for Hopx and Nppa were detected in adult mice hearts at day 0, 3, 7, 14, and 21 after sham (n=3) or TAC (n=3) surgery by qRT-PCR.
Figure 2
Figure 2
HOPX mRNA is downregulated in end-stage heart failure patients: Box plot represents HOPX transcript levels, detected by either microarray analysis (A) or qRT-PCR (B), in non-failing, failing and LVAD supported human hearts.
See this image and copyright information in PMC

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