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. 2010 Mar 22;2(1):69-72.

Histologic examination of the eye of acid-sensing ion channel 1a knockout mice

James A Render, Katharine R Howe, Amanda M Wunsch, Silvia Guionaud, Peter J Cox, John A Wemmie

Histologic examination of the eye of acid-sensing ion channel 1a knockout mice

James A Render et al. Int J Physiol Pathophysiol Pharmacol. .

Abstract

Various subtypes of the acid sensing ion channel have been detected in the retina of rodents and other mammalian species, but the functional importance of this finding is not clearly understood. The purpose of the study was to determine if retinal degeneration was present in ASIC1a-/- mice. The eyes of ASIC1a-/- mice, heterozygote ASIC1a+/- mice, and wild type ASIC1a+/+ mice that were 5 or 22-27 weeks old were processed by routine histotech-nological methods and examined for histologic changes in the retina and other portions of the eye. Additional sections of eyes from ASIC1a-/- and ASIC1a+/+ mice were labeled with peanut agglutinin (PNA) to evaluate cone pho-toreceptors. The retinas of ASIC1a-/-, ASIC1a+/-, and ASIC1a+/+ mice at 5 or 22-27 weeks of age were unremarkable and no morphologic changes in cone photo receptors were detected. Additional findings detected in the eye of ASIC1a+/+ mice included swelling of lens fibers or cataract that were also detected in some of the ASIC1a-/- or ASIC1a+/- mice. Lenticular findings were not considered to be associated with an absence of ASIC1a.

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Figures

Figure 1
Figure 1
Histologic appearance of the central retina of mice at 5 weeks of age. (A) Normal appearing central retina of an ASIC1a+/+ mouse. (B) Normal appearing central retina of an ASIC1a-/- mouse. H&E stain. Bar = 50 |jm. ASIC1a+/- mice also had normal appearing central retina histology (not shown).
Figure 2
Figure 2
Histologic appearance of the central retina of mice. (A) Unremarkable central retina of an ASIC1a+/+ mouse at 23 weeks. (B) Unremarkable central retina of an ASIC1a-/- mouse at 22 weeks. H&E stain. Bar = 50 μm. ASIC1a+/- mice had similar appearance (not shown).
Figure 3
Figure 3
Immunohistochemical staining of cone photo receptors in the central retina of mice with PNA. (A) Unremarkable cones in the central retina of an ASIC1a+/+ mouse at 23 weeks. (B) Unremarkable cones in the central retina of an ASIC1a-/- mouse at 22 weeks. Bar = 50 μm.
Figure 4
Figure 4
Histologic appearance of the anterior lens of mice. (A) A focal, minimal, unilateral area of liquefaction and globule formation in the anterior cortex of the lens of an ASIC1a+/+ mouse at 23 weeks. (B) Slight swelling of lens fibers in the anterior cortex of the lens of ASIC1a-/- mouse at 27 weeks of age. H&E stain. Bar = 50 μm.
See this image and copyright information in PMC

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