Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Apr;87(8):3082-6.
doi: 10.1073/pnas.87.8.3082.

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein

M A Garcia-Blanco  1 , G J Anderson, J Beggs, P A Sharp
Affiliations

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein

M A Garcia-Blanco et al. Proc Natl Acad Sci U S A. 1990 Apr.

Abstract

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Genes Dev. 1989 Dec;3(12A):1874-86 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 May;78(5):2737-41 - PubMed
    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
    1. Cell. 1984 Jun;37(2):415-27 - PubMed

Publication types

MeSH terms