doi: 10.1016/S0022-2836(05)80124-X.
Integration host factor stimulates the phage lambda pL promoter
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- PMID: 2140136
- DOI: 10.1016/S0022-2836(05)80124-X
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Integration host factor stimulates the phage lambda pL promoter
J Mol Biol.
.
Abstract
Escherichia coli integration host factor (IHF) is a small dimeric protein that binds to a specific DNA consensus sequence and produces DNA bending. Transcription from the bacteriophage lambda pL promoter is stimulated three- to fourfold by IHF both in vivo and in vitro. IHF binds with high-affinity to two tandem sites located just upstream from the pL promoter and enhances the formation of RNA polymerase-promoter closed complexes. The rate of isomerization to open complex is not influenced by IHF. IHF may stimulate recognition of pL by one or more of several mechanisms: (1) by bending DNA; (2) by making protein-protein contacts with RNA polymerase; or (3) by occluding a competing promoter upstream from pL.
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