doi: 10.1039/c0cc05334a.
Epub 2011 Mar 15.
Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site
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- PMID: 21409251
- DOI: 10.1039/c0cc05334a
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Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site
Chem Commun (Camb).
.
Abstract
A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation through the formation of a β-hairpin around the ligation site.
© The Royal Society of Chemistry 2011
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