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. 2011 Mar 16:4:65.
doi: 10.1186/1756-0500-4-65.

Applicability of anatid and galliform microsatellite markers to the genetic diversity studies of domestic geese (Anser anser domesticus) through the genotyping of the endangered zatorska breed

Krzysztof Andres  1 , Ewa Kapkowska
Affiliations

Applicability of anatid and galliform microsatellite markers to the genetic diversity studies of domestic geese (Anser anser domesticus) through the genotyping of the endangered zatorska breed

Krzysztof Andres et al. BMC Res Notes. .

Abstract

Background: The lack of a sufficient number of molecular markers seriously limits the cognition of genetic relationships within and between populations of many species. Likewise, the genetic diversity of domestic goose (Anser anser domesticus), with a great number of breeds throughout the world, remains poorly understood at the molecular level.

Findings: Thirty-five goose, seventeen duck and eight chicken microsatellite primer pairs were screened for their utility in the cross-species amplification on DNA from 96 individuals of Zatorska breed of domestic geese. Twenty-seven of 42 amplifying primer pairs revealed length-polymorphic products, but three of them were difficult to score. Fifteen primer pairs amplifying the same length product across all individuals. One polymorphic microsatellite locus was assigned by genotyping of known sex individuals to the Z-chromosome.

Conclusions: We present a set of 24 polymorphic microsatellite markers useful for population genetic studies of the domestic goose. Another 15 markers were classified as monomorphic, but they might also be suitable for the assessment of genetic diversity in geese.

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Figures

Figure 1
Figure 1
Proportion of polymorphic (P%), polymorphic but difficult to score (DS%), monomorphic (M%) and non-amplifying (UG%) markers in five marker sets in the study of genetic diversity of Zatorska geese APH, the set of 11 microsatellite loci isolated in domestic duck (Anas platyrhynchos domesticus) [22]; Bca μ, the set of 8 markers from Canada goose (Branta canadensis) [18]; CAUD-G, the set of 4 markers from domestic duck (Anas platyrhynchos domesticus) [17]; CKW, the set of 21 markers from swan goose (Anser cygnoides) [9,21]; TTUCG, the set of 5 markers from Canada goose (Branta canadensis) [25,35].
See this image and copyright information in PMC

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