A novel real-time polymerase chain reaction for identification of Salmonella enterica subspecies enterica

Abstract

Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identification of subspecies I, offering savings in time and labor over traditional methods.