2A to the fore - research, technology and applications

Abstract

The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence that mediates self-processing by a novel translational effect. Translation elongation arrest leads to release of the nascent polypeptide and re-initiation at the next in-frame codon. In this way discrete translation products are derived from a single open reading frame. Active 2A-like sequences have been found in (many) other viruses and trypanosome non-LTR retrotransposons. Exponential growth of 2A technology within the last decade has lead to many biotechnological/biomedical applications including the generation of transgenic plants/animals and genetic manipulation of human embryonic stem cells (hESCs).