In vitro expression profiling of myostatin, follistatin, decorin and muscle-specific transcription factors in adult caprine contractile myotubes

Abstract

Skeletal muscle is one of the several adult postmitotic tissues that retain the capacity to regenerate, which relies on a population of quiescent precursors, termed satellite cells. Proliferation and differentiation of myoblasts to form mature myotubes in vitro has been a valuable tool in the characterization of the cellular events during myogenesis, which is a multistep process starting with progenitor cell proliferation, followed by their exit from the cell cycle, differentiation, alignment, and fusion to form multinucleated myotubes. A typical feature during muscle differentiation is the variation in expression of various genes along with myogenic factors. In this experiment, mRNA level of myostatin, follistatin, decorin and three muscle-specific transcription factors in adult caprine contractile myotubes have been studied through quantitative real time PCR. We observed that the expression level of myostatin, decorin, Myf5 and myogenin transcripts were significantly higher in contractile myotubes compared to myoblast monolayer (P < 0.05), and follistatin level was similar in both types of cells, whereas MyoD transcript level was significantly high in monolayer culture which might be due heterogeneity of myoblast population. It is concluded that the information generated would provide the base line information as well as monitoring markers to undertake experiments aimed at modulating muscle growth.