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. 2011 Jun;55(6):2937-41.
doi: 10.1128/AAC.01670-10. Epub 2011 Mar 21.

First report of Klebsiella oxytoca strain coproducing KPC-2 and IMP-8 carbapenemases

Bin Li  1 , Jing-Yong Sun, Qing-Zhong Liu, Li-Zhong Han, Xin-Hong Huang, Yu-Xing Ni
Affiliations

First report of Klebsiella oxytoca strain coproducing KPC-2 and IMP-8 carbapenemases

Bin Li et al. Antimicrob Agents Chemother. 2011 Jun.

Abstract

The study shows for the first time the presence of the Klebsiella oxytoca strain fp10 coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 μg/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that the bla(KPC-2) gene was located on transferable plasmid pFP10-1 (∼54 kb), whereas the bla(IMP-8) gene was on transferable plasmid pFP10-2 (∼180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6 and ISKpn8 are involved in the spread of the bla(KPC-2) gene, while the transposable elements IS26, intI1, and tniC might contribute to the dissemination of the bla(IMP-8) gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-β-lactamase gene in Enterobacteriaceae from China.

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Figures

Fig. 1.
Fig. 1.
Electrophoretic profiles of plasmids (A) and hybridization with a blaKPC-2-specific (B) or a blaIMP-8-specific (C) probe. Lanes 1, 6, and 11, λ-HindIII-digested DNA (New England Biolabs, Beverly, MA); lanes 5, 10, and 15, marker (E. coli V517); lane 2, transconjugant harboring plasmid pFP10-2; lane 3, transconjugant harboring plasmids pFP10-1 and pFP10-2; lane 4, K. oxytoca strain fp10.
Fig. 2.
Fig. 2.
(a) Schematic representation of the genetic structure involved in the blaIMP-8 gene in plasmid pFP10-2. (b) Schematic representation of the genetic environment of the blaKPC-2 gene in plasmid pFP10-1 compared to that found around the blaKPC-2 gene in plasmid pKP048. The diagram of pKP048 is drawn based on the nucleotide sequences available in GenBank under accession number FJ628167.
See this image and copyright information in PMC

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