Effects of phenolic compounds of fermented thai indigenous plants on oxidative stress in streptozotocin-induced diabetic rats

Abstract

We investigated the effects of antioxidant activity of fermentation product (FP) of five Thai indigenous products on oxidative stress in Wistar rats with streptozotocin (STZ)-induced diabetes type II. The rats were fed with placebo and with the FP (2 and 6 mL/kg body weight/day) for 6 weeks. Rutin, pyrogallol and gallic acid were main compounds found in the FP. Plasma glucose levels in diabetic rats receiving the higher dose of the FP increased less when compared to the diabetic control group as well as the group receiving the lower FP dose (13.1%, 29%, and 21.1%), respectively. A significant dose-dependent decrease in plasma levels of thiobarbituric acid reactive substance (P < .05) was observed. In addition, the doses of 2 and 6 mL FP/kg/day decreased the levels of erythrocyte ROS in diabetic rats during the experiment, but no difference was observed when compared to the untreated diabetic rat group. Results imply that FP decreased the diabetes-associated oxidative stress to a large extent through the inhibition of lipid peroxidation. The FP also improved the abnormal glucose metabolism slightly but the difference was not statistically significant. Thus, FP may be a potential therapeutic agent by reducing injury caused by oxidative stress associated with diabetes.

Figure 1

HPLC/UV absorption analysis of the FP. Eluents of the FP were compared with the phenolic standards: rutin, gallic acid, pyrogallol, catechin, and caffeic acid as previously mentioned in the Method Section.

Figure 2

GC/MS identification of active ingredients of the FP (top) and of standard gallic acid (bottom).

Figure 3

Effect of the FP on body weight of STZ-induced diabetic rats. Data are expressed as mean ± SEM. ^# P < .05 when compared with the normal control group.

Figure 4

Effect of the FP on plasma glucose concentrations of STZ-induced diabetic rats. Data are expressed as mean ± SEM. ^# P < .05 when compared with the normal control group.

Figure 5

Effect of the FP on oxidative stress status of STZ-induced diabetic rats. Data are expressed as mean ± SEM. ^# P < .05 when compared with the normal control group, *P < .05 when compared with the diabetic group (DM).