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. 2011 Mar 22:11:103.
doi: 10.1186/1471-2407-11-103.

Impact of PINCH expression on survival in colorectal cancer patients

Jasmine Lööf  1 , Johan Rosell, Charlotte Bratthäll, Siv Doré, Hans Starkhammar, Hong Zhang, Xiao-Feng Sun
Affiliations

Impact of PINCH expression on survival in colorectal cancer patients

Jasmine Lööf et al. BMC Cancer. .

Abstract

Background: The adaptor protein PINCH is overexpressed in the stroma of several types of cancer, and is an independent prognostic marker in colorectal cancer. In this study we further investigate the relationship of PINCH and survival regarding the response to chemotherapy in colorectal cancer.

Results: Paraffin-embedded tissue sections from 251 primary adenocarcinomas, 149 samples of adjacent normal mucosa, 57 samples of distant normal mucosa and 75 lymph node metastases were used for immunohistochemical staining. Stromal staining for PINCH increased from normal mucosa to primary tumour to metastasis. Strong staining in adjacent normal mucosa was related to worse survival independently of sex, age, tumour location, differentiation and stage (p = 0.044, HR, 1.60, 95% CI, 1.01-2.52). PINCH staining at the invasive margin tended to be related to survival (p = 0.051). In poorly differentiated tumours PINCH staining at the invasive margin was related to survival independently of sex, age and stage (p = 0.013, HR, 1.90, 95% CI, 1.14-3.16), while in better differentiated tumours it was not. In patients with weak staining, adjuvant chemotherapy was related to survival (p = 0.010, 0.013 and 0.013 in entire tumour area, invasive margin and inner tumour area, respectively), but not in patients with strong staining. However, in the multivariate analysis no such relationship was seen.

Conclusions: PINCH staining in normal adjacent mucosa was related to survival. Further, PINCH staining at the tumour invasive margin was related to survival in poorly differentiated tumours but not in better differentiated tumours, indicating that the impact of PINCH on prognosis was dependent on differentiation status.

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Figures

Figure 1
Figure 1
Immunohistochemical staining for PINCH. A) Weak PINCH immunohistochemical staining (yellow-brown colour) in distant normal mucosa. B) Moderate staining in the adjacent normal mucosa (top right corner of the picture) and strong staining in the primary tumour (lower left of the picture). C) Even stronger staining in lymph node metastasis.
Figure 2
Figure 2
PINCH expression in distant and adjacent normal mucosa, primary tumour and lymph node metastasis. The frequency of the strong PINCH expression is significantly increased from adjacent normal mucosa to primary tumour (p = 0.0001) and from primary tumour to metastasis (p = 0.007).
Figure 3
Figure 3
PINCH expression in adjacent normal mucosa in relation to patient survival. Strong expression of PINCH in adjacent normal mucosa is significantly related to worse survival (p = 0.0008).
Figure 4
Figure 4
PINCH expression at the primary tumour invasive margin in relation to patient survival. A) Poorly differentiated tumours. B) Better differentiated tumours. Strong expression of PINCH is related to worse survival in poorly differentiated tumours (p = 0.001) but not in better differentiated tumours (p = 0.40).
See this image and copyright information in PMC

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