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. 2011 Jul;16(4):232-8.
doi: 10.1007/s12199-010-0191-9. Epub 2010 Nov 25.

Effect of habitual exercise on renal carcinogenesis by ferric nitrilotriacetate

Toyohiro Kato  1 , Hiroaki Kawaguchi, Noriaki Miyoshi, Kohji Aoyama, Masaharu Komatsu, Masahisa Horiuchi, Hiroki Yoshida, Toru Takeuchi
Affiliations

Effect of habitual exercise on renal carcinogenesis by ferric nitrilotriacetate

Toyohiro Kato et al. Environ Health Prev Med. 2011 Jul.

Abstract

Objectives: We investigated whether habitual exercise (HE) (treadmill running) suppresses development of renal cell carcinoma (RCC) induced by ferric nitrilotriacetate (Fe-NTA).

Methods: Male Fischer 344 rats were divided into six groups: group I, saline treatment (12 weeks = initiation period) and non-HE; group II, Fe-NTA treatment (12 weeks) and non-HE; group III, saline treatment and short-term (12 weeks) HE; group IV, Fe-NTA treatment and short-term HE; group V, saline treatment and long-term (40 weeks) HE; and group VI, Fe-NTA treatment and long-term HE. Saline treatment groups did not develop RCC, therefore we investigated the effects of HE among Fe-NTA treatment groups.

Results: Gross nodules (diagnosed as RCC), RCC represented by microcarcinomas (Mcs), karyomegalic cells (KCs), and degenerative tubules (DTs) were seen in rats treated with Fe-NTA. The number of Mcs, KCs, and DTs were increased in the short-term HE group when compared with those in the non-HE group, but were decreased in the long-term HE group when compared with those in the short-term HE group.

Conclusions: Short-term (initiation period) HE promoted renal carcinogenesis induced by Fe-NTA; however, long-term HE after the initiation period suppressed the promoted carcinogenesis.

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Figures

Fig. 1
Fig. 1
Summary of the experimental protocol
Fig. 2
Fig. 2
a White nodule (down arrow) induced in kidney by Fe-NTA in a group II rat. b Renal cell carcinoma induced in kidney by Fe-NTA in a group VI rat. c Renal cell microcarcinoma (down arrow) induced in kidney by Fe-NTA in a group VI rat. d Karyomegalic cell (down arrow) induced in proximal tubule by Fe-NTA in a group II rat. e Degenerative tubule with eosinophilic cytoplasm (down arrows), atypia (filled inverted triangle), and karyomegalic cell (open inverted triangle) induced in kidney by Fe-NTA in a group II rat. f Brown droplets (down arrows) in proximal tubule in a group II rat. g PCNA-positive nuclei in a renal cell carcinoma in a group II rat. h PCNA-positive nuclei (down arrows) in some proximal tubule epithelial cells in a group VI rat. i TUNEL-positive nuclei (down arrow) in some proximal tubule epithelial cells in a group VI rat. Bar 100 μm (c), 50 μm (e, g–i), 20 μm (b, d, f). H&E stain: b–f; Immunohistochemistry for PCNA (g, h) and apoptotic cells by TUNEL (i)
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