Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jul;3(2):106-12.
doi: 10.1007/BF02931793.

Stimulation of hydrogen peroxide production by drinking water contaminants in HL-60 cells sensitized by retinoic acid

H Yoshida  1 , S Inoue, K Yoshida, O Nakajima, S Mizuno
Affiliations

Stimulation of hydrogen peroxide production by drinking water contaminants in HL-60 cells sensitized by retinoic acid

H Yoshida et al. Environ Health Prev Med. 1998 Jul.

Abstract

Chemical carcinogens, such as chloroform and trichloroethylene, are present in drinking water in Japan. As these contaminants are believed to have a role in carcinogenesis, we examined if chloroform and trichloroethylene, as well as methylene chloride, xylene, benzene, and ethanol, have the ability to generate hydrogen peroxide (H(2)O(2)) in human polymorphonuclear leukocytes (PMN) and human leukemia (HL-60) cells. Methylene chloride, benzene, xylene, trichloroethylene, and ethanol did not increase cellular H(2)O(2): production as measured by flow cytometry nor as observed by confocal laser microscopy. In PMN and RAuntreated HL-60 cells chloroform did not significantly affect H(2)O(2) levels. However, in HL-60 cells sensitized by pretreatment of 10 nM retinoic acid (RA) for 12 h, chloroform induced a significant increase in H(2)O(2), but the increase induced by trichloroethylene was not significant. The observed increase in fluorescence was confirmed using a confocal laser microscope. These results indicate that chloroform and trichloroethylene may stimulate H(2)O(2) production in HL60 cells sensitized by pretreatment of RA. Our method may be useful to test if weak stimulants can stimulate intracellular H(2)O(2) production.

Keywords: Carcinogenicity; Differentiated leukemia cell; Flow cytometry; Hydrogen peroxide; Retinoic acid.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. None
    2. Ugawa M, Nakamura S, Nishimura H, Otake T. A study on safety evaluation for drinking water and control of organic compounds on water supply. I. safety evaluation for organic compounds in municipal drinking water. Jpn J Toxicol Environ Health 1993;39: 421–30.
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '1627912', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/1627912/'}]}
    2. El-Dib MA, Ali RK. Trihalomethanes in drinking water of Cairo, Egypt. Bull Environ Contamin Toxicol 1992;48: 378–86. - PubMed
    1. None
    2. Harris RH. The implication of cancer-causing substances in Mississippi river water. Environmental Defense Fund, Washington, D. C. 1974.
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '25299382', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/25299382/'}]}
    2. National Cancer Institute. Report on the carcinogenesis bioassay of chloroform. National Technical Information Service, PB-264018, Springfield, Virginia 1976. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '8137314', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/8137314/'}]}
    2. Rosin M, Anwar W, Ward A. Inflammation, chromosomal instability, and cancer: The schistosomiasis model. Cancer Res Suppl 1994;54: 1929s-33s. - PubMed