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. 2010 May;15(3):188-93.
doi: 10.1007/s12199-009-0126-5. Epub 2010 Jan 20.

Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

Chuancheng Wu  1 , Yuquan Lu, Kanehisa Morimoto
Affiliations

Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

Chuancheng Wu et al. Environ Health Prev Med. 2010 May.

Abstract

Objective: Results from previous studies suggest that alcohol consumption can be genotoxic on peripheral lymphocytes. The aim of our study was to examine the association of alcohol consumption and its genotoxic effect on hematopoietic stem cells in vivo.

Methods: We investigated 156 healthy Japanese males in a cross-sectional study. Lifestyles, including alcohol drinking behavior and cigarette smoking status, were investigated by means of a self-completed questionnaire. Polymorphisms of ADH1B and ALDH2 were identified by PCR-restriction fragment length polymorphism (RFLP) analysis. The presence of micronuclei in transferrin-positive reticulocytes (MN-RET) was detected with a single-laser flow cytometer. Associations between the genetic polymorphisms, lifestyle factors, and MN-RET frequency were statistically analyzed.

Results: We found a significant difference in the mean frequencies of MN-RET between habitual drinkers and non-habitual drinkers (P = 0.043), and between the ALDH2*1/*1 and ALDH2*2/*2 genotype (P = 0.015). The ADH1B*2 and ALDH2*2 haplotype was estimated to have a significantly higher influence on MN-RET frequency than the ADH1B*2 and ALDH2*1 haplotype (P = 0.00035), and the frequency of alcohol consumption played a significant role in MN-RET frequency on the background of the ADH1B*2 and ALDH2*1 haplotype (P = 0.012).

Conclusion: The results of our study suggest a possible association between the ADH1B and ALDH2 polymorphism and the genotoxic effects of alcohol drinking on hematopoietic stem cells.

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References

    1. Norppa H. Cytogenetic markers of susceptibility: influence of polymorphic carcinogen-metabolizing enzymes. Environ Health Perspect. 1997;105(Suppl 4):829–835. doi: 10.2307/3433290. - DOI - PMC - PubMed
    1. Iarmarcovai G, et al. Micronuclei frequency in peripheral blood lymphocytes of cancer patients: a meta-analysis. Mutat Res. 2008;659(3):274–283. doi: 10.1016/j.mrrev.2008.05.006. - DOI - PubMed
    1. Goetz R, Sram RJ, Dohnalova J. Relationship between experimental results in mammals and man. I. Cytogenetic analysis of bone marrow injury induced by a single dose of cyclophosphamide. Mutat Res. 1975;31(4):247–254. - PubMed
    1. Jensen MK, Nyfors A. Cytogenetic effects of methotrexate on human cells in vivo: comparison between results obtained by chromosome studies on bone-marrow cells and blood lymphocytes and by the micronucleus test. Mutat Res. 1979;64(5):339–343. - PubMed
    1. Schlegel R, MacGregor JT, Everson RB. Assessment of cytogenetic damage by quantitation of micronuclei in human peripheral blood erythrocytes. Cancer Res. 1986;46(7):3717–3721. - PubMed

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