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. 2011 May-Jun;58(3):269-71.
doi: 10.1111/j.1550-7408.2011.00541.x. Epub 2011 Mar 24.

Development of a nested PCR for environmental detection of the pathogenic free-living amoeba Balamuthia mandrillaris

Arine F Ahmad  1 , Peter W Andrew, Simon Kilvington
Affiliations

Development of a nested PCR for environmental detection of the pathogenic free-living amoeba Balamuthia mandrillaris

Arine F Ahmad et al. J Eukaryot Microbiol. 2011 May-Jun.

Abstract

A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.

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