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. 1978 Sep;14(9):746-53.
doi: 10.1007/BF02617967.

Isolation and identification of epithelial-like cells in culture by a collagenase-separation technique

R J Kanoza, D M Brunette, A D Purdon, J Sodek

Isolation and identification of epithelial-like cells in culture by a collagenase-separation technique

R J Kanoza et al. In Vitro. 1978 Sep.

Abstract

An operational criterion for the identification and isolation of epithelial-like (E) cells, based on their ability to cover and protect a collagen gel from the action of collagenase, has been developed. The E cells isolated by this collagenase-separation technique (CST) exhibited the ultrastructural features, including desmosomes and abundant tonofilaments, that are considered characteristic of this cell type. Unlike confluent cultures of fibroblast-like (F) cells, E cells were not found to have large external transformation-sensitive (LETS) protein on their surface membranes. The CST provides a nondestructive and efficient means of identifying and isolating E cells from mixed populations.

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