Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis

Abstract

Objective: To test the hypothesis that the expression of the different isoforms of the estrogen receptor alpha (ER-α) and beta (ER-β) and the progesterone receptor A (PR-A) and B (PR-B) would be differentially modulated in uteri with adenomyosis compared with controls and that modulation would be related to the menstrual cycle.

Design: Case control, blinded comparison.

Setting: University department.

Patient(s): 54 premenopausal women with and 35 without uterine adenomyosis as the sole pathology.

Intervention(s): Multiple samples studied using immunohistochemistry for estrogen and progesterone receptors.

Main outcome measure(s): Histomorphometric analysis of receptor expression.

Result(s): The ER-α expression in the adenomyotic endometrium was different from that of the normal endometrium and the foci in the midsecretory phase of the cycle, but expression of ER-α in the inner and outer myometrium was not statistically significantly different. The ER-β expression was statistically significantly elevated in the adenomyotic functionalis gland during the proliferative phase and throughout the myometrium across the entire menstrual cycle. Expression of PR-A was similar to that of PR-B, with reduced expression in the basalis stroma, and inner and outer myometrium in the adenomyotic samples. The pattern of ER-β, PR-A, and PR-B expression was similar in the endometrial basalis and adenomyotic foci.

Conclusion(s): These data suggest ER-β expression and the lack of PR expression are related to the development and/or progression of adenomyosis and might explain the poor response of adenomyosis-associated menstrual symptoms to progestational agents.