Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar 30:11:41.
doi: 10.1186/1471-2377-11-41.

Selected acute phase CSF factors in ischemic stroke: findings and prognostic value

Maia Beridze  1 , Tamar Sanikidze, Roman Shakarishvili, Nino Intskirveli, Natan M Bornstein
Affiliations

Selected acute phase CSF factors in ischemic stroke: findings and prognostic value

Maia Beridze et al. BMC Neurol. .

Abstract

Background: Study aimed at investigation of pathogenic role and prognostic value of several selected cerebrospinal fluid acute phase factors that can reflect the severity of ischemic brain damage.

Methods: Ninety five acute ischemic stroke patients were investigated. Ischemic region visualized at the twenty fourth hour by conventional Magnetic Resonance Imaging. Stroke severity evaluated by National Institute Health Stroke Scale. One month outcome of disease was assessed by Barthel Index. Cerebrospinal fluid was taken at the sixth hour of stroke onset. CSF pro- and anti-inflammatory cytokines were studied by Enzyme Linked Immunosorbent Assay. Nitric Oxide and Lipoperoxide radical were measured by Electron Paramagnetic Resonance. CSF Nitrate levels were detected using the Griess reagent. Statistics performed by SPSS-11.0.

Results: At the sixth hour of stroke onset, cerebrospinal fluid cytokine levels were elevated in patients against controls. Severe stroke patients had increased interleukin-6 content compared to less severe strokes (P < 0.05). Cerebrospinal fluid Electron Paramagnetic Resonance signal of nitric oxide was increased in patients against controls. Severe stroke group had an elevated Electron Paramagnetic Resonance signal of lipoperoxiradical compared to less severe stroke. Cerebrospinal fluid nitrate levels in less severe stroke patients were higher than those for severe stroke and control. Positive correlation was established between the initial interleukin-6 content and ischemic lesion size as well as with National Institute Health Stroke Scale score on the seventh day. Initial interleukin-6 and nitrate levels in cerebrospinal fluid found to be significant for functional outcome of stroke at one month.

Conclusion: According to present study the cerebrospinal fluid contents of interleukin-6 and nitrates seem to be the most reliable prognostic factors in acute phase of ischemic stroke.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Comparison of EPR signal intensity of CSF free NO between the study groups and control. Box plots represent mean values (SD). P < 0.05 between the Group1, Group 2 and control.
Figure 2
Figure 2
Comparison of EPR signal intensity of CSF free LOO- between the study groups and control. Box plots represent mean values (SD). P < 0.05 between the study groups and control. P < 0.50 between the Group 1 and Group 2.
Figure 3
Figure 3
Relation of initial CSF IL-6 content with ischemic lesion size 24 hours from stroke. After all acute phase factors and risk factors entered into the stepwise model of multivariate logistic regression only initial CSF IL-6 gained significance and positively correlated with mean predicted probability of brain ischemic lesion size (r = +0.34; P < 0.05).
See this image and copyright information in PMC

References

    1. Aktas O, Ullrich O, Infante-Duarte C, Nitsch R, Zipp F. Neuronal damage in brain inflammation. Arch Neurol. 2007;64(2):185–189. doi: 10.1001/archneur.64.2.185. - DOI - PubMed
    1. Cherubini A, Ruggiero C, Polidori MC, Mecocci P. Potential markers of oxidative stress in stroke. Free Rad Biol Med. 2005;39:841–852. doi: 10.1016/j.freeradbiomed.2005.06.025. - DOI - PubMed
    1. The European Stroke Organization (ESO) Executive Committee and the ESO Writing Committee. Guidelines for Management of Ischaemic Stroke and Transient Ischaemic Attack. Cerebrovasc Dis. 2008;25:457–507. doi: 10.1159/000131083. - DOI - PubMed
    1. Adams HP, Bendixen BH, Kapelle LJ. the TOAST investigators. Classification of subtypes of acute ischemic stroke. Definitions for use of multicenter clinical trial TOAST. Stroke. 1993;24:35–41. - PubMed
    1. Jood K, Redfors P, Rosengren A, Blomstrand CH, Jern CH. Self-perceived psychological stress and ischemic stroke: a case-control study. BMC Medicine. 2009;7:53. doi: 10.1186/1741-7015-7-53. - DOI - PMC - PubMed

Publication types