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Review
. 2011 Apr;8(4 Suppl):S6-11.
doi: 10.1038/nmeth.1557.

Development and applications of single-cell transcriptome analysis

Fuchou Tang  1 , Kaiqin Lao, M Azim Surani
Affiliations
Review

Development and applications of single-cell transcriptome analysis

Fuchou Tang et al. Nat Methods. 2011 Apr.

Abstract

Dissecting the relationship between genotype and phenotype is one of the central goals in developmental biology and medicine. Transcriptome analysis is a powerful strategy to connect genotype to phenotype of a cell. Here we review the history, progress, potential applications and future developments of single-cell transcriptome analysis. In combination with live cell imaging and lineage tracing, it will be possible to decipher the full gene expression network underlying physiological functions of individual cells in embryos and adults, and to study diseases.

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Figures

Figure 1
Figure 1
Strategies for single cell transcriptome analysis. Solid lines represent strategies that have been demonstrated experimentally; dotted lines represent proposed strategies that will probably be realized in the near future.
See this image and copyright information in PMC

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