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. 2011 Mar 31;2(3):e139.
doi: 10.1038/cddis.2011.23.

BAG3 is required for IKKα nuclear translocation and emergence of castration resistant prostate cancer

M Ammirante, V De Laurenzi, V Graziano, M C Turco, A Rosati

BAG3 is required for IKKα nuclear translocation and emergence of castration resistant prostate cancer

M Ammirante et al. Cell Death Dis. .
No abstract available

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Figures

Figure 1
Figure 1
(a) myc-CaP cells derived from the FVB background were provided by C Sawyers (University of California, Los Angeles and Memorial Sloan Kettering Cancer Center). Virus-containing supernatants were added to the cells for 2 days with polybrene, and transduced cells were selected in 5 μg/ml puromycin (Invitrogen, Carlsbad, CA, USA). Male FVB mice were injected with myc-CaP cells, previously infected with lentiviruses expressing control (Scrambled) or BAG3-specific siRNAs. When tumors reached 900 mm3, mice (n=10 per group) were castrated. Tumor size was measured every 2–3 days. Results are expressed as means±S.D. Data were analyzed by Student's t-test using GraphPad Prism statistical program (*P<0.001). (b) Paraffin sections of tumors transduced with BAG3 siRNA (right panel) or control vector (left panel) as described in a were stained using a specific antibody recognizing IKKα (Imgenex, San Diego, CA, USA) and counterstained with hematoxylin as previously described (original magnification × 200). (c) myc-CaP cells were infected with a lentivirus carrying a control (Scrambled; left panel) or a BAG3-specific siRNA (right panel) then treated with LTα2β1, TGFβ or RANKL, all at 10 ng/ml (Sigma, Aldrich, St. Louis, MO, USA) for 1 h. Cells were harvested and subjected to nuclei/cytosol fractionation. Lysates were analyzed by immunoblot, using the same antibody as in b (C, cytosol; N, nucleus).
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References

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