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. 2011 Apr 1;2011(4):pdb.prot5594.
doi: 10.1101/pdb.prot5594.

Preparation of postimplantation mouse embryos for imaging

Monica D Garcia, Ryan S Udan, Anna-Katerina Hadjantonakis, Mary E Dickinson

Preparation of postimplantation mouse embryos for imaging

Monica D Garcia et al. Cold Spring Harb Protoc. .
No abstract available

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Figures

FIGURE 1.
FIGURE 1.
Dissection of an E9.5 mouse embryo. The uterine horn is removed from the mother and separated into individual embryos surrounded by the uterus and deciduum (A–C). The uterus is peeled away (D), leaving the embryo surrounded by a thick decidual layer (E). The deciduum is then removed (F) and the Reichert’s membrane/trophoblast layer (G,H) is carefully separated from the embryo, exposing the intact embryo with the yolk sac available for live imaging (I).
See this image and copyright information in PMC

References

    1. Garcia MD, Udan RS, Hadjantonakis A-K, Dickinson ME. 2011a. Live imaging of mouse embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.top104. - DOI - PMC - PubMed
    1. Garcia MD, Udan RS, Hadjantonakis A-K, Dickinson ME. 2011b. Preparation of rat serum for culturing mouse embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5593. - DOI - PMC - PubMed
    1. Garcia MD, Udan RS, Hadjantonakis A-K, Dickinson ME. 2011c. Time-lapse imaging of postimplantation mouse embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5595. - DOI - PMC - PubMed
    1. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2003. Manipulating the mouse embryo: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

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