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. 2011 May;8(5):409-12.
doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.

A more efficient method to generate integration-free human iPS cells

Keisuke Okita  1 , Yasuko Matsumura, Yoshiko Sato, Aki Okada, Asuka Morizane, Satoshi Okamoto, Hyenjong Hong, Masato Nakagawa, Koji Tanabe, Ken-ichi Tezuka, Toshiyuki Shibata, Takahiro Kunisada, Masayo Takahashi, Jun Takahashi, Hiroh Saji, Shinya Yamanaka
Affiliations
Free article

A more efficient method to generate integration-free human iPS cells

Keisuke Okita et al. Nat Methods. 2011 May.
Free article

Abstract

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

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Comment in

  • Keeping things simple.
    Rao M. Rao M. Nat Methods. 2011 May;8(5):389-90. doi: 10.1038/nmeth.1598. Nat Methods. 2011. PMID: 21527929 No abstract available.

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