[Bacteria diversity of soil of Populus Euphratica forest in the hinterland of Taklimakan desert]

Abstract

Objective: The aim of study was to investigate bacterial diversity of Populus Euphratica forest in the hinterland of Taklimakan desert. All the isolateds were be used as inoculants for silage and biofertilizer.

Methods: Strains were isolated by culture-dependent method. Gram staining, NaCl tolerance,enzyme activity (including amylase, esterase, cellulase) were determined by strand methods. Phylogenetic trees based on 16S rRNA gene sequences were constructed by using the neighbour-joining.

Results: A total of 27 strains were obtained. Phylogenetic analysis of the bacterial 16S rRNA gene showed that all isolates fell into one of the following four bacterial lineages: Actinobacteria (16 strains), Proteobacteria (4 strains), Firmicutes (6 strains) and Bacteroidetes (1 strains). Gram staining indicated that 5 strains were gram-negative and the others were gram-positive. Among these, 15 strains showed amylase activity, 9 strains showed esterase activity and 9 strains showed cellulase activity. All strains growth occurred at in presence of 2% NaCl, 22 strains growth occurred at in presence of 5% NaCl and only 1 strain tolerated up to 15% NaCl.

Conclusion: The bacterial population diversity is abundant in soil of Populus Euphratica Forest in the hinterland of Taklimakan Desert, which is worthy of further investigation.