Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Apr;17(4):691-4.
doi: 10.3201/eid1704.101726.

Rapid genotyping of swine influenza viruses

Polly W Y Mak  1 , Chloe K S Wong, Olive T W Li, Kwok Hung Chan, Chung Lam Cheung, Edward S Ma, Richard J Webby, Yi Guan, Joseph S Malik Peiris, Leo L M Poon
Affiliations

Rapid genotyping of swine influenza viruses

Polly W Y Mak et al. Emerg Infect Dis. 2011 Apr.

Abstract

The emergence of pandemic (H1N1) 2009 virus highlighted the need for enhanced surveillance of swine influenza viruses. We used real-time reverse-transcription PCR-based genotyping and found that this rapid and simple genotyping method may identify reassortants derived from viruses of Eurasian avian-like, triple reassortant-like, and pandemic (H1N1) 2009 virus lineages.

PubMed Disclaimer

Figures

Figure
Figure
Genotyping of A) polymerase acidic protein, B) hemagglutinin, and C) neuraminidase segments of A/swine/Hong Kong/201/2010 influenza (H1N1) virus. Black line, amplification signal of SYBR green dye; blue line, amplification signal of cyanine 5 dye; green line, threshold level. The x-axis denotes the cycle number of a quantitative PCR assay, and the y-axis denotes the fluorescence intensity over the background.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, et al.. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med. 2009;360:2605–15. 10.1056/NEJMoa0903810 - DOI - PubMed
    1. Pasma T, Joseph T. Pandemic (H1N1) 2009 infection in swine herds, Manitoba, Canada. Emerg Infect Dis. 2010;16:706–8. - PMC - PubMed
    1. Pereda A, Cappuccio J, Quiroga MA, Baumeister E, Insarralde L, Ibar M, et al. Pandemic (H1N1) 2009 outbreak on pig farm, Argentina. Emerg Infect Dis. 2010;16:304–7. - PMC - PubMed
    1. Howden KJ, Brockhoff EJ, Caya FD, McLeod LJ, Lavoie M, Ing JD, et al. An investigation into human pandemic influenza virus (H1N1) 2009 on an Alberta swine farm. Can Vet J. 2009;50:1153–61. - PMC - PubMed
    1. Poon LL, Mak PW, Li OT, Chan KH, Cheung CL, Ma ES, et al. Rapid detection of reassortment of pandemic (H1N1) 2009 influenza virus. Clin Chem. 2010;56:1340–4. 10.1373/clinchem.2010.149179 - DOI - PMC - PubMed

Publication types

LinkOut - more resources