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. 2011 Jun;105(6):1115-8.
doi: 10.1160/TH10-09-0628. Epub 2011 Apr 7.

Phospholipid binding lowers immunogenicity of human recombinant factor VIII in von Willebrand factor knockout mice

D S Pisal, S V Balu-Iyer

Phospholipid binding lowers immunogenicity of human recombinant factor VIII in von Willebrand factor knockout mice

D S Pisal et al. Thromb Haemost. 2011 Jun.
No abstract available

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Figures

Figure 1
Figure 1
Effect of PI and PS on immunogenicity of FVIII. A) The mean of total antibody titers (bars) and individual (closed circles) antibody titers against FVIII as determined by antibody capture ELISA in vWF mice immunized via i.v. administration of free FVIII, FVIII-PI, and FVIII-PS. B) The mean of inhibitory titers (bars) and individual (closed circles) antibody titers against FVIII as determined by the Nijmegen modification of the Bethesda assay in vWF knockout mice immunized via i.v. administration of free FVIII, FVIII-PI, FVIII-PS. C) Left panel shows the raw data for Thymidine (3H) incorporation (counts per minute) of different groups of splenocytes. The mean count (cross) and splenocytes from individual spleen (n=6) are shown (filled triangles). Typically negative group showed counts less than 300. Right panel shows the stimulation indices of different groups of splenocytes. The mean stimulation index (cross) and individual (filled circles) stimulation indices from individual spleens (n=6) of different treatment groups as determined by thymidine (3H) incorporation T cell proliferation assay.
Figure 1
Figure 1
Effect of PI and PS on immunogenicity of FVIII. A) The mean of total antibody titers (bars) and individual (closed circles) antibody titers against FVIII as determined by antibody capture ELISA in vWF mice immunized via i.v. administration of free FVIII, FVIII-PI, and FVIII-PS. B) The mean of inhibitory titers (bars) and individual (closed circles) antibody titers against FVIII as determined by the Nijmegen modification of the Bethesda assay in vWF knockout mice immunized via i.v. administration of free FVIII, FVIII-PI, FVIII-PS. C) Left panel shows the raw data for Thymidine (3H) incorporation (counts per minute) of different groups of splenocytes. The mean count (cross) and splenocytes from individual spleen (n=6) are shown (filled triangles). Typically negative group showed counts less than 300. Right panel shows the stimulation indices of different groups of splenocytes. The mean stimulation index (cross) and individual (filled circles) stimulation indices from individual spleens (n=6) of different treatment groups as determined by thymidine (3H) incorporation T cell proliferation assay.
Figure 1
Figure 1
Effect of PI and PS on immunogenicity of FVIII. A) The mean of total antibody titers (bars) and individual (closed circles) antibody titers against FVIII as determined by antibody capture ELISA in vWF mice immunized via i.v. administration of free FVIII, FVIII-PI, and FVIII-PS. B) The mean of inhibitory titers (bars) and individual (closed circles) antibody titers against FVIII as determined by the Nijmegen modification of the Bethesda assay in vWF knockout mice immunized via i.v. administration of free FVIII, FVIII-PI, FVIII-PS. C) Left panel shows the raw data for Thymidine (3H) incorporation (counts per minute) of different groups of splenocytes. The mean count (cross) and splenocytes from individual spleen (n=6) are shown (filled triangles). Typically negative group showed counts less than 300. Right panel shows the stimulation indices of different groups of splenocytes. The mean stimulation index (cross) and individual (filled circles) stimulation indices from individual spleens (n=6) of different treatment groups as determined by thymidine (3H) incorporation T cell proliferation assay.
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