Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Oct;5(10):3479-89.
doi: 10.1093/nar/5.10.3479.

The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones

C Spadafora, P Oudet, P Chambon
Free PMC article

The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones

C Spadafora et al. Nucleic Acids Res. 1978 Oct.
Free PMC article

Abstract

The four histones H2A, H2B, H3 and H4 from calf thymus, CHO and sea urchin gastrula cells were associated by stepwise dialysis from 2 M NaCl with SV40 DNA Form I. The in vitro-assembled chromatins were visualized by electron microscopy and the size of the DNA fragments generated by digestion with DNase II was determined. Irrespective of the origin of the histones, the size of the smallest DNA band generated at early times of digestion was about 190 base pairs, whereas oligomeric DNA bands were multiples of 140 bp. These results support our previous proposal that the four histones H2A, H2B, H3 and H4 are able to organize more than 140 bp of DNA, but do not provide any evidence that the variability of histones H2A and H2B plays a role in the variability of the DNA repeat length of native chromatins.

PubMed Disclaimer

References

    1. Cell. 1977 Feb;10(2):237-43 - PubMed
    1. Nucleic Acids Res. 1976 Nov;3(11):3173-92 - PubMed
    1. Eur J Biochem. 1978 Feb;83(2):615-28 - PubMed
    1. Cell. 1977 Apr;10(4):633-40 - PubMed
    1. J Mol Biol. 1977 Jan 25;109(3):393-404 - PubMed

LinkOut - more resources