Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Controlled Clinical Trial
. 2011 May;38(5):434-41.
doi: 10.1111/j.1600-051X.2011.01706.x.

Salivary biomarkers of periodontal disease in response to treatment

William M Sexton  1 , Yushun Lin, Richard J Kryscio, Dolphus R Dawson 3rd, Jeffrey L Ebersole, Craig S Miller
Affiliations
Controlled Clinical Trial

Salivary biomarkers of periodontal disease in response to treatment

William M Sexton et al. J Clin Periodontol. 2011 May.

Abstract

Background: Salivary biomarkers of periodontitis were assessed longitudinally to determine response to therapy.

Methods: A 6-month case-controlled study of adults with chronic periodontitis was performed, with 33 participants receiving oral hygiene instructions (OHI) alone and 35 with scaling and root planing (SRP) combined with OHI. Saliva samples collected at week 0, 16 and 28 were analysed for interleukin (IL)-1β, IL-8, macrophage inflammatory protein (MIP)-1α, matrix metalloproteinase-8 (MMP-8), osteoprotegerin (OPG), and tumour necrosis factor-α (TNF)-α. Clinical measures of periodontal disease were recorded at each visit.

Results: All parameters of periodontal health improved significantly in both groups by week 16 (p<0.0001) with the SRP group demonstrating greater benefit at week 16 and 28. Baseline OPG and TNF-α levels changed significantly at both follow-up visits (p<0.03), regardless of treatment group. IL-1β and MMP-8 levels decreased significantly from baseline (p<0.04) in the SRP group only. OPG, MMP-8, and MIP-1α were significantly reduced in responders compared with non-responders (p=0.04, 0.01, 0.05, respectively). In receiver-operating characteristic analyses, MMP-8 produced the highest area under the curve (0.7; p=0.01).

Conclusion: Salivary levels of IL-1β, MMP-8, OPG, and MIP-1α reflected disease severity and response to therapy suggesting their potential utility for monitoring periodontal disease status.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest

The authors report no conflicts of interest related to this study.

Figures

Figure 1
Figure 1
Clinical periodontal measures by group and visit. A) OHI and B) SRP. Significant reductions (p<0.0001) occurred in each parameter compared to baseline within groups as determined by ANCOVA.
Figure 2
Figure 2
Boxplots of biomarkers by group and visit. A) IL-1β, B) IL-8, C) MIP-1α, D) MMP-8, E) OPG, and F) TNF-α. Left three boxes correspond to OHI and right three boxes for SRP. Horizontal lines represent the 25th, 50th and 75th percentiles, plus sign represents the mean, and filled circles represent outliers and * indicates outliers clipped from boxplot. X-axis corresponds with weeks.
Figure 3
Figure 3
Boxplots of change scores of biomarkers (log values) at week 28 by group and responder status. Left two boxes correspond to non-responder and right boxes to responders. Significant p-values for comparing responders to non-responders are indicated on the plots.
See this image and copyright information in PMC

References

    1. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4:1–6. - PubMed
    1. Armitage GC. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004;34:9–21. - PubMed
    1. Bertolini DR, Nedwin GE, Bringman TS, Smith DD, Mundy GR. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986;319:516–518. - PubMed
    1. Buduneli N, Biyikoğlu B, Sherrabeh S, Lappin DF. Saliva concentrations of RANKL and osteoprotegerin in smoker versus non-smoker chronic periodontitis patients. J Clin Periodontol. 2008;35:846–852. - PubMed
    1. Choi SJ, Cruz JC, Craig F, Chung H, Devlin RD, Roodman GD, Alsina M. Macrophage inflammatory protein 1-alpha is a potential osteoclast stimulatory factor in multiple myeloma. Blood. 2000;96:671–675. - PubMed

Publication types

MeSH terms