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Multicenter Study
. 2011 Jun;49(6):2259-61.
doi: 10.1128/JCM.02250-10. Epub 2011 Apr 13.

Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay

Phyllis Della-Latta  1 , Hossein Salimnia, Theresa Painter, Fann Wu, Gary W Procop, Deborah A Wilson, Wendy Gillespie, Alayna Mender, Benjamin S Crystal
Affiliations
Multicenter Study

Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay

Phyllis Della-Latta et al. J Clin Microbiol. 2011 Jun.

Abstract

A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).

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