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Editorial
. 2011 Jul;301(1):C12-5.
doi: 10.1152/ajpcell.00100.2011. Epub 2011 Apr 13.

Interpretation of ³¹P NMR saturation transfer experiments: what you can't see might confuse you. Focus on "Standard magnetic resonance-based measurements of the Pi→ATP rate do not index the rate of oxidative phosphorylation in cardiac and skeletal muscles"

R S Balaban, A P Koretsky
Editorial

Interpretation of ³¹P NMR saturation transfer experiments: what you can't see might confuse you. Focus on "Standard magnetic resonance-based measurements of the Pi→ATP rate do not index the rate of oxidative phosphorylation in cardiac and skeletal muscles"

R S Balaban et al. Am J Physiol Cell Physiol. 2011 Jul.
No abstract available

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Conflict of interest statement

DISCLOSURES

No conflicts of interest, financial or otherwise, are declared by the author(s).

Figures

Fig. 1.
Fig. 1.
This is adapted from Jucker et al. (; copyright 2000 National Academy of Sciences, U.S.A.). The top tracing is a 31P NMR spectrum from the human leg under control conditions. The bottom spectrum is during the saturation of the γ-ATP phosphate where the signal is eliminated, or saturated, by the irradiation, as indicated by the arrow. The saturation of the γ-ATP results in a decrease in the Pi resonance, as shown by the two bars over the Pi peak placed by Jucker et al. This has been ascribed to the ATP hydrolysis rate. However, nearly equal decreases in signal are seen for the α-ATP (ΔM α-ATP) and β-ATP (ΔM β-ATP) resonance, as illustrated by the bars we have added. The reasons for these decreases in signal with the γ-ATP irradiation are discussed within the article. PCr, phosphocreatine; M, magnetization; ppm, parts per million.
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References

    1. Alger JR , den Hollander JA , Shulman RG. In vivo phosphorus-31 NMR saturation transfer studies of ATPase kinetics in Saccharomyces cerevisiae. Biochemistry 21: 2957–2963, 1982. - PubMed
    1. Alger JR , Shulman RG. NMR studies of enzymatic rates in vitro and in vivo by magnetization transfer. Q Rev Biophys 17: 83–124, 1984. - PubMed
    1. Balaban RS , Kantor HL , Ferretti JA. In vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phosphorous NMR. J Biol Chem 258: 12787–12789, 1983. - PubMed
    1. Balaban RS , Kantor HL , Ferretti JA. In vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phosphorous NMR. J Biol Chem 258: 12787–12789, 1983. - PubMed
    1. Balaban RS , Knepper MA. Nitrogen-14 nuclear magnetic resonance spectroscopy of mammalian tissues. Am J Physiol Cell Physiol 245: C439–C444, 1983. - PubMed

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