Association of TRB3 Q84R polymorphism with polycystic ovary syndrome in Chinese women

Abstract

Background: Tribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation. A single nucleotide polymorphism located in the second extron of the human TRB3 gene is thought to be associated with insulin resistance. The latter is a core abnormality in PCOS independent of obesity. The present study was designed to clarify the relationships of TRB3 Q84R polymorphism with PCOS in a Chinese women group.

Methods: A case-control study with two groups: PCOS group (n = 336) and control group of infertility women for tubal and/or male factor (n = 116) was performed. Genotyping of the TRB3 R84 variant was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results: The frequency of genotype QQ in PCOS women was significantly lower, while genotype QR and RR were significantly higher than that in control group (p < 0.05). However, the difference disappeared after adjustment for BMI. At glucose1h, glucose2h and insulin2h point, the difference between QQ individuals and R84 allele carriers in PCOS women reached statistical significance during OGTT (p < 0.05).

Conclusions: TRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women.

Figure 1

The representative electrophoretic gel featuring the RFLP analysis of TRB3 Q84R polymorphism. The PCR products were digested with MspІ followed by 2% agarose gel electrophoresis: the resulting wild-type (QQ) samples contained one band sized 593 bp, heterozygotes (QR) samples contained two bands sized 593 bp and 297 bp and homozygotes (RR) contained one band sized 297 bp. M: 100 bp DNA marker; 1: RR genotype; 2,3,5,9,11,12: QQ genotype; 4,6,7,8,10: QR genotype.