Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii
- PMID: 2149344
- DOI: 10.1016/0378-1097(90)90350-y
Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii
Abstract
Dithiothreitol-dependent MgATP2- and Mg-ADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically in tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.
Similar articles
-
Functional Analysis of the Role of Toxoplasma gondii Nucleoside Triphosphate Hydrolases I and II in Acute Mouse Virulence and Immune Suppression.Infect Immun. 2016 Jun 23;84(7):1994-2001. doi: 10.1128/IAI.00077-16. Print 2016 Jul. Infect Immun. 2016. PMID: 27091930 Free PMC article.
-
The relationship between nucleoside triphosphate hydrolase (NTPase) isoform and Toxoplasma strain virulence in rat and human toxoplasmosis.Microbes Infect. 2003 Jul;5(9):797-806. doi: 10.1016/s1286-4579(03)00148-5. Microbes Infect. 2003. PMID: 12850206
-
Membrane localization and demonstration of isoforms of nucleoside triphosphate hydrolase from Toxoplasma gondii.Parasitology. 2001 Jan;122 Pt 1:15-23. doi: 10.1017/s0031182000007101. Parasitology. 2001. PMID: 11197760
-
Heat shock protein 70 is a potential virulence factor in murine toxoplasma infection via immunomodulation of host NF-kappa B and nitric oxide.J Immunol. 2002 Jul 15;169(2):958-65. doi: 10.4049/jimmunol.169.2.958. J Immunol. 2002. PMID: 12097402
-
Biochemical and molecular characterization of nucleoside triphosphate hydrolase isozymes from the parasitic protozoan Toxoplasma gondii.J Biol Chem. 1995 May 12;270(19):11391-7. doi: 10.1074/jbc.270.19.11391. J Biol Chem. 1995. PMID: 7744775
Cited by
-
Knocking Down TcNTPDase-1 Gene Reduces in vitro Infectivity of Trypanosoma cruzi.Front Microbiol. 2020 Mar 18;11:434. doi: 10.3389/fmicb.2020.00434. eCollection 2020. Front Microbiol. 2020. PMID: 32256481 Free PMC article.
-
A novel GDP-dependent pyruvate kinase isozyme from Toxoplasma gondii localizes to both the apicoplast and the mitochondrion.J Biol Chem. 2008 May 16;283(20):14041-52. doi: 10.1074/jbc.M709015200. Epub 2008 Mar 6. J Biol Chem. 2008. PMID: 18326043 Free PMC article.
-
Human platelet inhibition of Toxoplasma gondii growth.Clin Exp Immunol. 1998 Feb;111(2):325-33. doi: 10.1046/j.1365-2249.1998.00499.x. Clin Exp Immunol. 1998. PMID: 9486399 Free PMC article.
-
High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii.J Clin Microbiol. 1992 May;30(5):1291-3. doi: 10.1128/jcm.30.5.1291-1293.1992. J Clin Microbiol. 1992. PMID: 1316368 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
