Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009;17 Suppl(spe):13-8.
doi: 10.1590/s1678-77572009000700004.

Resistance to tetracycline and β-lactams and distribution of resistance markers in enteric microorganisms and pseudomonads isolated from the oral cavity

Marcelle Marie Buso Ramos  1 , Ellen Cristina Gaetti-Jardim, Elerson Gaetti-Jardim Junior
Affiliations

Resistance to tetracycline and β-lactams and distribution of resistance markers in enteric microorganisms and pseudomonads isolated from the oral cavity

Marcelle Marie Buso Ramos et al. J Appl Oral Sci. 2009.

Abstract

This study evaluated the occurrence of enteric bacteria and pseudomonads resistant to tetracycline and β-lactams in the oral cavity of patients exhibiting gingivitis (n=89), periodontitis (n=79), periodontally healthy (n=50) and wearing complete dentures (n=41). Microbial identification and presence of resistance markers associated with the production of β-lactamases and tetracycline resistance were performed by using biochemical tests and PCR. Susceptibility tests were carried out in 201 isolates of enteric cocci and rods. Resistance to ampicillin, amoxicillin/clavulanic acid, imipenem, meropenem and tetracycline was detected in 57.4%, 34.6%, 2.4%, 1.9% and 36.5% of the isolates, respectively. β-lactamase production was observed in 41.2% of tested microorganisms, while the most commonly found β-lactamase genetic determinant was gene blaTEM. Tetracycline resistance was disseminated and a wide scope of tet genes were detected in all studied microbial genus.

PubMed Disclaimer

References

    1. Aminov RI, Garrigues-Jeanjean N, Mackie RI. Molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins. Appl Environ Microbiol. 2001;67:22–32. - PMC - PubMed
    1. Barbosa FCB, Mayer MPA, Saba-Chujfi E, Cai S. Subgingival occurrence and antimicrobial susceptibility of enteric rods and pseudomonads from Brazilian periodontitis patients. Oral Microbiol Immunol. 2001;16:306–310. - PubMed
    1. Belaaouaj A, Lapoumeroulie C, Caniça MM, Névot GVP, Krishnamoorthy R, Paul G. Nucleotide sequences of the genes coding for the TEM-like β-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2) FEMS Microbiol Lett. 1994;120:75–80. - PubMed
    1. Daniluk T, Fiedoruk K, Œciepuk M, Zaremba ML, Rozkiewicz D, Cylwik-Rokicka D, et al. Aerobic bacteria in the oral cavity of patients with removable dentures. Adv Med Sci. 2006;51:86–90. - PubMed
    1. Gaetti-Jardim E, Jr, Landucci LF, Lins AS, Vieira EMM, Oliveira SR. Susceptibility of strict and facultative anaerobes isolated from endodontic infections to metronidazole and β-lactams. J Appl Oral Sci. 2007;15:539–545. - PMC - PubMed

Publication types

MeSH terms