Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jul;39(1):185-91.
doi: 10.3892/ijo.2011.1006. Epub 2011 Apr 14.

The level of secretory leukocyte protease inhibitor is decreased in metastatic head and neck squamous cell carcinoma

Christian Cordes  1 , Robert Häsler, Christian Werner, Tibor Görögh, Christoph Röcken, Lena Hebebrand, W Martin Kast, Markus Hoffmann, Stefan Schreiber, Petra Ambrosch
Affiliations

The level of secretory leukocyte protease inhibitor is decreased in metastatic head and neck squamous cell carcinoma

Christian Cordes et al. Int J Oncol. 2011 Jul.

Abstract

Head and neck squamous cell carcinomas (HNSCC) represent the sixth largest group among all human malignancies. However, the exact molecular mechanisms inducing the genesis and the progression of metastasis in these tumors are poorly understood. The identification of molecular alterations involved in metastasis of HNSCC might influence the value of clinical diagnostics, impact therapy strategies and finally improve the prognosis of the patients. The purpose of this study was to identify clinically relevant alterations at the transcriptional and translational levels, when comparing metastatic (N+) and non-metastatic (N0) primary HNSCC. Three transcripts HERPUD1, SLPI and RAD51 were selected for further validation based on their association with carcinogenesis and metastasis. Quantitative real-time-PCR was performed to determine the mRNA expression levels. For subsequent confirmation of the results, immunohistochemistry was performed applying a monoclonal anti-SLPI antibody on 121 HNSCC tumor specimens (N0, n=40; N+, n=81). In metastatic primary cancer, SLPI mRNA showed 5.9-fold lower expression in comparison with non-metastatic primary cancer (p=0.0092). Immunohistochemical staining revealed a fold change of -1.79 between the N+ and the N0 group (p=0.0002). The results presented here clearly indicate the repression of SLPI, measurable on both, mRNA and protein levels in metastatic primary HNSCC as compared to non-metastatic HNSCC. Therefore, it can be assumed that SLPI might have a substantial protective effect on the metastasis process of HNSCC.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Immunohistochemistry of SLPI in HNSCC. (A) no immunoreactivity, (B) weak immunoreactivity, (C) moderate immunoreactivity and (D) strong immunoreactivity.
Figure 2
Figure 2
Result of the qRT-PCR analysis. SLPI is significantly lower expressed in metastatic HNSCC.
Figure 3
Figure 3
Results of the immunohistochemical staining with the monoclonal anti-SLPI antibody. One hundred and twenty-one samples are divided into N+ and N0. A statistically significant difference is observed between the groups (p=0.00027).
See this image and copyright information in PMC

References

    1. Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. CA Cancer J Clin. 2005;55:74–108. - PubMed
    1. Akervall J. Gene profiling in squamous cell carcinoma of the head and neck. Cancer Metastasis Rev. 2005;24:87–94. - PubMed
    1. Chin D, Boyle GM, Porceddu S, Theile DR, Parsons PG, Coman WB. Head and neck cancer: past, present and future. Expert Rev Anticancer Ther. 2006;6:1111–1118. - PubMed
    1. Pantel K, Brakenhoff RH. Dissecting the metastatic cascade. Nat Rev Cancer. 2004;4:448–456. - PubMed
    1. Robbins KT, Clayman G, Levine PA, et al. Neck dissection classification update: revisions proposed by the American Head and Neck Society and the American Academy of Otolaryngology-Head and Neck Surgery. Arch Otolaryngol Head Neck Surg. 2002;128:751–758. - PubMed

Publication types

Substances