Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens
- PMID: 21508148
- PMCID: PMC3122740
- DOI: 10.1128/JCM.02431-10
Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens
Abstract
We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
References
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- Baker S. E., et al. 2010. Extranasal methicillin-resistant Staphylococcus aureus colonization at admission to an acute care Veterans Affairs Hospital. Infect. Control Hosp. Epidemiol. 31:42–46 - PubMed
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- BD Diagnostics 4 April 2011, accession date BD GeneOhm MRSA assay. Package insert. BD Diagnostics, Quebec, Quebec, Canada: http://www.bd.com/geneohm/english/products/pdfs/mrsa_pkginsert.pdf
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- Collett D. 1991. Modelling binary data. Chapman & Hall, London, United Kingdom
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