Microanalytical RPC-5 columns for rapid chromatography of aminoacyl-tRNAs
- PMID: 215093
Microanalytical RPC-5 columns for rapid chromatography of aminoacyl-tRNAs
Abstract
A microanalytical reversed-phase chromatographic system for the separation of aminoacyl-tRNA's on microbore (0.3cm X 25cm) columns is described. Chromatography of lysyl-tRNA from polyoma virus transformed mouse fibroblasts revealed at least seven identifiable isoacceptors. The system has the advantages of speed, sensitivity and excellent resolution of isoaccepting tRNA's.