Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Sep 15;174(3):699-702.
doi: 10.1042/bj1740699.

A simple assay for adenylate cyclase in intact cells by affinity elution chromatography

A K Sinha, R W Colman

A simple assay for adenylate cyclase in intact cells by affinity elution chromatography

A K Sinha et al. Biochem J. .

Abstract

A method using the principle of affinity elution chromatography is described for the assay of adenylate cyclase in intact human platelets. By incubating platelet-rich plasma in the presence of radioactively labelled adenine, the ATP pool of the cells was prelabelled. Formation of labelled cyclic AMP from ATP was determined by extracting the platelets with HC1O4. After removal of the latter as KC1O4, the extract containing cyclic AMP and other adenine nucleotides was adsorbed in a NN-diethyl-N-2-hydroxypropylamino (QAE)-cellulose column. The column was washed, and subsequently cyclic AMP was specifically eluted with a cyclic AMP-dependent protein kinase and the radioactivity of the eluate was determined.

PubMed Disclaimer

References

    1. Recent Prog Horm Res. 1965;21:623-46 - PubMed
    1. Pharmacol Rev. 1966 Mar;18(1):237-41 - PubMed
    1. J Biol Chem. 1977 May 25;252(10):3310-4 - PubMed
    1. Experientia. 1977 Mar 15;33(3):301-2 - PubMed
    1. Eur J Biochem. 1977 Mar 1;73(2):367-71 - PubMed